Biooo

Joaquin B. Agony, Glacial S. Beanie (14 August 2014) (21 August 2014) l. Introduction Microscopes and the field of knowledge are dickens inseparable things. Almost all Sciences are associated with the drop of a microscope, a device use to view objects which are not visible to the naked eye. The term microscope came from the Greek term Micros which means junior-grade and Spoken which means to examine (Vocabulary. Com, 2014).Today, microscopes are of great importance particularly on the field of Biological Science. One of its major use is to get word the sizes of microorganisms which are of great importance in to a lower placestanding certain biologic phenomena. Getting the actual size of microscopic objects such as microorganisms employ calibrated eyepiece micron is termed micrometer (Echoic, et. Al. , 2000). It is important for investigators across a number of discip melodys. A biologist, for example, needs to know the exact size of two organisms to provide an intelligent c omparison (I. E.Almoner). The intent of this activity is to create an knowingness in proper handling techniques ND correct use of a microscope to students pursuing courses in the field of Biological Sciences. This activity is also intend to teach students microscope normalisation techniques. II. Materials and Methods To able to obtain the exact measurement of a specimen, the optical micrometer should be calibrated first. By doing so, the calibration immutable (C) is obtained. To get the calibration constant, a put micrometer, an ocular micrometer and a microscope is used.The ocular micrometer was placed inside the ocular lens of the microscope and the dot micrometer on the stage. The field was adjusted so that a musical note in the ocular micrometer is superimposing a line in the stage micrometer. Once this was set, the number of divisions in both micrometers from the first superimposing lines to the next line that superimposed was counted. The number of divisions in the stage micrometer was divided by the divisions in the ocular micrometer. The quotient was multiplied by pm.The product obtained was the calibration constant. The calibration constant was determined in each of the object glass lenses of the microscope. Once the ocular micrometer was calibrated, the pessimism were viewed and measured. The correct measurement was obtained by counting the ocular micrometer divisions occupied by the specimen and multiplying it by the calibration constant of the objective lens used. The deviation between the measurement should only appear on the decimal places of the figure. Ill. Results and Discussion Table 1. Calibration Constant below disparate Microscope Objective Lenses Table 1. 0 shows the summary for the computed calibration constant under the high power objective (HOP) and the the low power objective (LOOP). Under the HOP, the umber of divisions in the ocular micrometer between the two superimposing lines is quadruplet while on the stage micrometer it is five. Dividing five by four, the harbor 1. 25 is obtained. This is not yet the calibration constant. The value 1. 25 should be multiplied by pm to get the C.